6BRG
The SAM domain of mouse SAMHD1 is critical for its activation and regulation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.582, 109.919, 162.089 |
| Unit cell angles | 90.00, 105.78, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.24528 |
| Rwork | 0.244 |
| R-free | 0.27769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aja |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.164 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.150 | |
| Number of reflections | 34596 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 98.8 | |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.4 | 298 | 100 mM SPG (Qiagen) buffer, pH 7.4, 25% PEG 1500 |
