6BQF
Pol II elongation complex with 'dT-AP' at i+1, i-1 position
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-18 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.400, 221.350, 193.700 |
Unit cell angles | 90.00, 100.60, 90.00 |
Refinement procedure
Resolution | 82.763 - 3.350 |
R-factor | 0.2184 |
Rwork | 0.217 |
R-free | 0.24460 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.534 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.800 | 3.530 |
High resolution limit [Å] | 3.350 | 3.350 |
Number of reflections | 92041 | |
<I/σ(I)> | 6.5 | |
Completeness [%] | 99.9 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 50mM dioxane, 10mM DTT, 9-12% PEG6000 |