6BP4
Structure of the S. pombe Clr4 catalytic domain bound to SAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.919, 71.040, 96.674 |
Unit cell angles | 90.00, 125.06, 90.00 |
Refinement procedure
Resolution | 59.349 - 2.770 |
R-factor | 0.2248 |
Rwork | 0.223 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mvh |
RMSD bond length | 0.006 |
RMSD bond angle | 0.585 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.140 | 2.984 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.125 | 0.712 |
Rpim | 0.084 | 0.480 |
Number of reflections | 14482 | 1958 |
<I/σ(I)> | 6.5 | |
Completeness [%] | 77.2 | |
Redundancy | 2.94 | |
CC(1/2) | 0.995 | 0.328 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M magnesium formate dihydrate and 20 % PEG 3350 |