6BOK
E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 210.820, 450.450, 615.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.550 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 3.750 |
High resolution limit [Å] | 3.550 | 3.550 |
Number of reflections | 699120 | |
<I/σ(I)> | 7.9 | |
Completeness [%] | 100.0 | |
Redundancy | 38.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride. |
2 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride. |
3 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride. |
4 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride. |
5 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride. |
5 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride. |