6BOG

Crystal structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription

Replaces:  3DMQ

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97935,0.97951
Spacegroup name	P 43
Unit cell lengths	123.856, 123.856, 187.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.193 - 3.205
R-factor	0.25
Rwork	0.244
R-free	0.27890
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.310
High resolution limit [Å]	3.200	6.870	3.200
Rmerge	0.123	0.061	0.966
Rmeas	0.139	0.069	1.112
Rpim	0.064	0.032	0.539
Total number of observations	203007
Number of reflections	45885	4515	4534
<I/σ(I)>	10.5
Completeness [%]	99.0	95.9	98.5
Redundancy	4.4	4.5	3.8
CC(1/2)		0.995	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	Ammonium sulfate, 1,4-Dioxane, Ethylene glycol, MES