6BNT

Crystal structure of AP2 mu1 adaptin C-terminal domain with IRS-1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97914
Spacegroup name	P 64
Unit cell lengths	125.334, 125.334, 74.818
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.642 - 3.200
R-factor	0.2092
Rwork	0.206
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bxx
RMSD bond length	0.011
RMSD bond angle	1.231
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.600	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.161	0.292
Number of reflections	11485	574
<I/σ(I)>	13.2
Completeness [%]	100.0
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.0 M sodium malonate, pH 5.0, 0.1 M sodium acetate tri-hydrate, pH 4.5, 2% w/v PEG20000