6BNT
Crystal structure of AP2 mu1 adaptin C-terminal domain with IRS-1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97914 |
Spacegroup name | P 64 |
Unit cell lengths | 125.334, 125.334, 74.818 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.642 - 3.200 |
R-factor | 0.2092 |
Rwork | 0.206 |
R-free | 0.23430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bxx |
RMSD bond length | 0.011 |
RMSD bond angle | 1.231 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.600 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.161 | 0.292 |
Number of reflections | 11485 | 574 |
<I/σ(I)> | 13.2 | |
Completeness [%] | 100.0 | |
Redundancy | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.0 M sodium malonate, pH 5.0, 0.1 M sodium acetate tri-hydrate, pH 4.5, 2% w/v PEG20000 |