6BNL
Crystal structure of TCR-MHC-like molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.666, 150.318, 100.144 |
| Unit cell angles | 90.00, 96.12, 90.00 |
Refinement procedure
| Resolution | 38.070 - 2.600 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3quz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.010 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.049 | 0.375 |
| Number of reflections | 71925 | 10545 |
| <I/σ(I)> | 21.2 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 18-20% PEG3350 8% tacsimate pH 5.0 0.5% dioxane |
