6BMK
Crystal structure of MHC-I like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.566, 71.549, 104.747 |
| Unit cell angles | 90.00, 101.78, 90.00 |
Refinement procedure
| Resolution | 44.740 - 2.430 |
| R-factor | 0.2187 |
| Rwork | 0.217 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cd1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.770 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rpim | 0.312 | |
| Number of reflections | 32070 | |
| <I/σ(I)> | 17.88 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 294 | 100 mM BisTris (pH 6-6.5), 200 mM CaCl2 and 21-26% PEG 3350 |
