6BMI

Crystal Structure of GltPh R397C in complex with L-Serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953700
Spacegroup name	P 61
Unit cell lengths	114.750, 114.750, 322.336
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.900
R-factor	0.2279
Rwork	0.227
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nwx
RMSD bond length	0.008
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.480	4.270
High resolution limit [Å]	3.900	3.900
Rmerge	0.063	0.778
Rmeas	0.088
Rpim	0.063	0.778
Number of reflections	20718	15254
<I/σ(I)>	8.5	1.9
Completeness [%]	94.9	95.8
Redundancy	3.1	3
CC(1/2)	0.992	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	50 mM citric acid 50 mM Disodium phosphate 100 mM Lithium sulfate 15-32% PEG1000