6BMI
Crystal Structure of GltPh R397C in complex with L-Serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953700 |
| Spacegroup name | P 61 |
| Unit cell lengths | 114.750, 114.750, 322.336 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.900 |
| R-factor | 0.2279 |
| Rwork | 0.227 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nwx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.480 | 4.270 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.063 | 0.778 |
| Rmeas | 0.088 | |
| Rpim | 0.063 | 0.778 |
| Number of reflections | 20718 | 15254 |
| <I/σ(I)> | 8.5 | 1.9 |
| Completeness [%] | 94.9 | 95.8 |
| Redundancy | 3.1 | 3 |
| CC(1/2) | 0.992 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 50 mM citric acid 50 mM Disodium phosphate 100 mM Lithium sulfate 15-32% PEG1000 |
