6BLX

Crystal structure of IAg7 in complex with insulin mimotope p8G9E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979500
Spacegroup name	P 1 21 1
Unit cell lengths	39.347, 112.626, 62.176
Unit cell angles	90.00, 107.36, 90.00

Refinement procedure

Resolution	40.849 - 2.323
R-factor	0.1702
Rwork	0.168
R-free	0.21980
RMSD bond length	0.007
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.300	2.320
Rmerge	0.080	0.460
Number of reflections	21452
<I/σ(I)>	8.7
Completeness [%]	96.3
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	18% PEG20000, 100mM sodium citrate at pH5.0