6BKU
Crystal Structure of the Human CAMKK2B bound to GSK650394
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.968610 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.971, 72.971, 119.301 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.000 |
R-factor | 0.19483 |
Rwork | 0.193 |
R-free | 0.23658 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uyj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.309 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.970 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.096 | 1.724 |
Rpim | 0.033 | 0.579 |
Number of reflections | 20267 | 1601 |
<I/σ(I)> | 12.5 | 1.4 |
Completeness [%] | 89.9 | 99.6 |
Redundancy | 10.3 | 10.4 |
CC(1/2) | 0.999 | 0.534 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 20% PEG smear medium, 0.2M sodium formate, 0.1M sodium-potassium phosphate, 10% glycerol |