6BKM
Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin E190D mutant apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 209.273, 131.447, 71.921 |
| Unit cell angles | 90.00, 97.88, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.18247 |
| Rwork | 0.181 |
| R-free | 0.20934 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fnk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.461 |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rpim | 0.050 | 0.440 |
| Number of reflections | 96961 | 9442 |
| <I/σ(I)> | 16.3 | 1.2 |
| Completeness [%] | 99.4 | 97.2 |
| Redundancy | 4.5 | 3.2 |
| CC(1/2) | 1.000 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M sodium cacodylate pH 6.5, 6% PEG 8000, and 39% 2-methyl-2,4-pentanediol |
