6BKC
Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 6a bound to broadly neutralizing antibody AR3B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.447, 59.437, 143.923 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 32.580 - 2.600 |
| R-factor | 0.2336 |
| Rwork | 0.231 |
| R-free | 0.27590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kbk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.490 |
| High resolution limit [Å] | 2.434 | 2.450 |
| Rpim | 0.060 | |
| Number of reflections | 20780 | 815 |
| <I/σ(I)> | 10.2 | 1.6 |
| Completeness [%] | 90.1 | 59.2 |
| Redundancy | 3.1 | 1.8 |
| CC(1/2) | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 20% (w/v) PEG 3500, 0.2M Li-chloride |
