6BKB
Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 6a bound to broadly neutralizing antibody AR3A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.922, 67.559, 218.353 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.382 - 2.799 |
R-factor | 0.2316 |
Rwork | 0.230 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mwf |
RMSD bond length | 0.003 |
RMSD bond angle | 0.573 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.598 | 2.600 |
Rpim | 0.030 | |
Number of reflections | 26290 | 998 |
<I/σ(I)> | 19.6 | 1 |
Completeness [%] | 91.7 | 46.2 |
Redundancy | 10.9 | 2.8 |
CC(1/2) | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 20% (w/v) PEG 3500, 0.2M sodium-citrate |