6BIY

HLA-DRB1 in complex with Histone 2B peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	67.826, 182.478, 76.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.223 - 2.050
R-factor	0.185114762639
Rwork	0.183
R-free	0.23185
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mdi
RMSD bond length	0.007
RMSD bond angle	0.866
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.220	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.053
Number of reflections	30243
<I/σ(I)>	9.9
Completeness [%]	99.6
Redundancy	4.4
CC(1/2)	0.989	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	294	20-28% PEG 3350, 0.2M Potassium Nitrate, 0.1M Bis-Tris-Propane pH 7.3