6BHY
Mouse Immunoglobulin G 2c Fc fragment with single GlcNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-08-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.233, 89.215, 69.851 |
Unit cell angles | 90.00, 132.20, 90.00 |
Refinement procedure
Resolution | 44.608 - 2.040 |
R-factor | 0.2132 |
Rwork | 0.210 |
R-free | 0.25360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rgs |
RMSD bond length | 0.019 |
RMSD bond angle | 1.873 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.238 | 2.113 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.093 | 0.553 |
Rmeas | 0.109 | 0.655 |
Number of reflections | 29903 | 2977 |
<I/σ(I)> | 8.95 | 1.75 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.7 | 3.3 |
CC(1/2) | 0.995 | 0.756 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291.15 | 50mM Tris pH 8 with 10% PEG 6K |