6BG2
Crystal structure of cGMP-dependent protein kinase Ialpha (PKG Ialpha) catalytic domain in AMP-PNP bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2015-09-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97741 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 165.390, 98.500, 116.424 |
Unit cell angles | 90.00, 133.47, 90.00 |
Refinement procedure
Resolution | 47.231 - 1.830 |
R-factor | 0.1884 |
Rwork | 0.188 |
R-free | 0.21550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fjq |
RMSD bond length | 0.007 |
RMSD bond angle | 0.782 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.230 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Number of reflections | 117904 | 5795 |
<I/σ(I)> | 16.8 | |
Completeness [%] | 99.0 | 98.3 |
Redundancy | 4.7 | 4.8 |
CC(1/2) | 0.999 | 0.698 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 23.2% PEG1500, 0.08 M PCTP, pH8.0, 0.5% w/v polyvinylpyrrolidone K15 |