6BFY

Crystal structure of enolase from Escherichia coli with bound 2-phosphoglycerate substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.1949
Spacegroup name	P 21 21 21
Unit cell lengths	105.940, 142.400, 206.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.610 - 1.810
R-factor	0.16892
Rwork	0.166
R-free	0.23223
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fym
RMSD bond length	0.026
RMSD bond angle	2.231
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.610	1.840
High resolution limit [Å]	1.810	1.810
Rmerge	0.116	0.607
Rmeas	0.132	0.702
Rpim	0.062	0.349
Number of reflections	283200	13929
<I/σ(I)>	6.1	1.5
Completeness [%]	100.0	99.9
Redundancy	4	3.9
CC(1/2)	0.991	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	2.0 M ammonium sulfate, 0.1 M MES, pH 6.0, 0.1 M sodium/potassium tartrate