6BFS

The mechanism of GM-CSF inhibition by human GM-CSF auto-antibodies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	P 1 21 1
Unit cell lengths	70.454, 48.129, 77.602
Unit cell angles	90.00, 99.86, 90.00

Refinement procedure

Resolution	40.000 - 2.000
R-factor	0.185
Rwork	0.182
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rjl
RMSD bond length	0.017
RMSD bond angle	1.842
Data scaling software	Aimless (0.5.4)
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	39.550	2.050
High resolution limit [Å]	2.000	8.950	2.000
Rmerge	0.074	0.031	0.613
Rmeas	0.087	0.037	0.714
Rpim	0.045	0.019	0.365
Number of reflections	34696	421	2557
<I/σ(I)>	12
Completeness [%]	99.4	98.4	99
Redundancy	3.7	3.4	3.8
CC(1/2)	0.998	0.998	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283.15	0. 2 M Sodium Chloride, 20% PEG 8000, 0.1 M phosphate Citrate 4.5