6BEK
Structure of sIHF bound to an 8bp palindromic DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.260, 72.110, 103.257 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.380 - 1.700 |
R-factor | 0.2025 |
Rwork | 0.201 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4itq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.163 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.064 | 0.366 |
Rpim | 0.028 | 0.162 |
Number of reflections | 35669 | 1740 |
<I/σ(I)> | 22.6 | 3.9 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 6.2 | 6 |
CC(1/2) | 0.977 | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 0.1 M HEPES pH 7.6, 0.2 M KSCN, 19% (w/v) PEG 3350, and 5% (v/v) ethylene glycol |