6BCR
Complex of 14-3-3 theta with an IRSp53 peptide phosphorylated at T340
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.007, 69.114, 84.560 |
| Unit cell angles | 105.25, 95.72, 115.04 |
Refinement procedure
| Resolution | 31.288 - 1.986 |
| R-factor | 0.1963 |
| Rwork | 0.195 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2br9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.290 | 2.060 |
| High resolution limit [Å] | 1.986 | 1.986 |
| Rmerge | 0.042 | 0.379 |
| Number of reflections | 76071 | 6527 |
| <I/σ(I)> | 21.8 | 3.5 |
| Completeness [%] | 95.7 | 92 |
| Redundancy | 3.4 | 2.7 |
| CC(1/2) | 0.999 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 0.15 M Magnesium Formate, 18% PEG3350, 4% TFE |
