6BCB
A Complex between PH Domain of p114RhoGEF and Activated RhoA Bound to a GTP Analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.934, 60.039, 65.475 |
| Unit cell angles | 90.00, 108.14, 90.00 |
Refinement procedure
| Resolution | 27.291 - 1.401 |
| R-factor | 0.1377 |
| Rwork | 0.136 |
| R-free | 0.16490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.343 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.291 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 70421 | 3332 |
| <I/σ(I)> | 23.5 | 2 |
| Completeness [%] | 99.6 | 95.1 |
| Redundancy | 4.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22-24% PEG 3350, 100mM Bis-Tris, 200mM NaCl |
