6BB4

Fab/epitope complex of mouse monoclonal antibody C5.2 targeting a phospho-tau epitope.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	161.156, 40.535, 228.064
Unit cell angles	90.00, 104.07, 90.00

Refinement procedure

Resolution	44.250 - 2.099
Rwork	0.218
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.250	2.174
High resolution limit [Å]	2.099	2.099
Rmerge	0.069	0.544
Rmeas	0.079	0.625
Rpim	0.039	0.303
Number of reflections	84763	8330
<I/σ(I)>	12.34	2.51
Completeness [%]	99.5	99.32
Redundancy	4.2	4.2
CC(1/2)	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	25.5% polyethylene glycol 4000, 0.17 M ammonium sulfate, 15% glycerol