6BAY
Stigmatella aurantiaca bacterial phytochrome P1, PAS-GAF-PHY T289H mutant, room temperature structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-12-09 |
Detector | MPCCD |
Wavelength(s) | 1.3 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 84.230, 84.230, 477.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.799 - 3.150 |
R-factor | 0.2248 |
Rwork | 0.222 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bap |
RMSD bond length | 0.012 |
RMSD bond angle | 1.594 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.800 | 3.200 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.011 | |
Number of reflections | 52483 | 1956 |
<I/σ(I)> | 4.76 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 399 | 107 |
CC(1/2) | 0.998 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 11 mg/mL protein in 0.1 M MES, pH 6.2, 8-5% w/v PEG20000, 5.2% v/v acetonitrile |