6BAQ
Mus musculus BPIFA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.200, 200.582, 89.499 |
| Unit cell angles | 90.00, 126.15, 90.00 |
Refinement procedure
| Resolution | 41.789 - 2.503 |
| R-factor | 0.222884893344 |
| Rwork | 0.221 |
| R-free | 0.27824 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kgh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | Coot (1.12_2829) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.800 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.071 |
| Number of reflections | 64175 |
| <I/σ(I)> | 24.8 |
| Completeness [%] | 98.9 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 310.15 | 20% PEG 1000, 0.2 M Lithium Sulfate, 0.1 M Phosphate-citrate pH 4.2 |
