6BA5
Potent and Selective Antitumor Activity of a T-Cell Engaging Bispecific Antibody Targeting a Membrane-Proximal Epitope of ROR1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 |
Unit cell lengths | 58.020, 73.900, 84.120 |
Unit cell angles | 89.86, 71.30, 84.49 |
Refinement procedure
Resolution | 39.530 - 1.620 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Antibody R11 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.850 | 1.650 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 0.087 | 0.497 |
Rmeas | 0.103 | 0.595 |
Rpim | 0.054 | 0.321 |
Number of reflections | 160894 | 7849 |
<I/σ(I)> | 8.5 | 2.5 |
Completeness [%] | 96.1 | 94.6 |
Redundancy | 3.7 | 3.3 |
CC(1/2) | 0.993 | 0.717 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 0.2 M Li Sulfate, 20% PEG3350 |