6B9Z
Trastuzumab Fab v3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.490, 105.290, 117.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.500 - 1.820 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ioi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.840 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.075 | 0.760 |
| Number of reflections | 59094 | |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 98.3 | 98 |
| Redundancy | 13.5 | 14 |
| CC(1/2) | 0.999 | 0.657 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris, pH 7.5, 24 mM NaCl, 15% PEG3350 |
