6B9O

Structure of GH 38 Jack Bean alpha-mannosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-09
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.92019
Spacegroup name	P 21 21 21
Unit cell lengths	67.305, 119.667, 277.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.550 - 1.841
R-factor	0.1771
Rwork	0.175
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o7d
RMSD bond length	0.008
RMSD bond angle	1.032
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.550	1.950
High resolution limit [Å]	1.840	1.840
Rmeas	0.177
Number of reflections	193407	57728
<I/σ(I)>	10.79	1.28
Completeness [%]	99.2	95.1
Redundancy	6.55	5.96
CC(1/2)	0.996	0.486

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2M tri-ammonium citrate, 20% w/v PEG 3350