6B9O
Structure of GH 38 Jack Bean alpha-mannosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.92019 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.305, 119.667, 277.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.550 - 1.841 |
| R-factor | 0.1771 |
| Rwork | 0.175 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o7d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.550 | 1.950 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmeas | 0.177 | |
| Number of reflections | 193407 | 57728 |
| <I/σ(I)> | 10.79 | 1.28 |
| Completeness [%] | 99.2 | 95.1 |
| Redundancy | 6.55 | 5.96 |
| CC(1/2) | 0.996 | 0.486 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M tri-ammonium citrate, 20% w/v PEG 3350 |
