6B9M
Crystal structure of UHRF1 TTD domain in complex with the polybasic region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97741 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.219, 66.142, 120.576 |
| Unit cell angles | 90.00, 104.92, 90.00 |
Refinement procedure
| Resolution | 28.760 - 1.680 |
| R-factor | 0.1834 |
| Rwork | 0.182 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.680 |
| Number of reflections | 69241 |
| <I/σ(I)> | 27.1 |
| Completeness [%] | 98.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Succinic acid pH7.0, 15% (v/v) Polyethylene glycol 3350 |
