6B9F

Human ATL1 mutant - F151S bound to GDPAlF4-

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-17
Detector	ADSC QUANTUM 210
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	49.613, 115.721, 184.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.684 - 1.900
R-factor	0.1787
Rwork	0.178
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ido
RMSD bond length	0.007
RMSD bond angle	0.865
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.000
High resolution limit [Å]	1.900
Rmerge	0.119
Rmeas	0.129
Number of reflections	83775
<I/σ(I)>	10.7
Completeness [%]	98.8
Redundancy	6.8
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M ammonium citrate tribasic pH 7, 0.1 M imidazole pH7, 20% PEG monomethyl ether 2,000, 1 mM GDP, 2 mM MgCl2, 2 mM AlCl2, 20 mM NaF