6B9E

Human ATL1 mutant - R77A / F151S bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.631
Spacegroup name	P 1
Unit cell lengths	52.062, 68.107, 76.016
Unit cell angles	116.85, 90.77, 98.50

Refinement procedure

Resolution	13.850 - 1.990
R-factor	0.2042
Rwork	0.203
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q5e
RMSD bond length	0.009
RMSD bond angle	0.973
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.750
High resolution limit [Å]	1.990
Rmerge	0.064
Rmeas	0.086
Number of reflections	60236
<I/σ(I)>	9.9
Completeness [%]	95.7
Redundancy	2
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M Bis-Tris pH 5.5, 0.2 M ammonium sulfate, 22% PEG3350, 1 mM GDP, 2 mM MgCl2