6B8O

WT Ig-like V Domain with Phosphatidylserine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-07-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00001
Spacegroup name	P 41 21 2
Unit cell lengths	160.331, 160.331, 86.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.360 - 2.200
R-factor	0.2236
Rwork	0.222
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ud7
RMSD bond length	0.019
RMSD bond angle	2.165
Data scaling software	Aimless (0.5.32)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.360	47.360	2.260
High resolution limit [Å]	2.200	9.590	2.200
Rmerge	0.106	0.034	1.733
Total number of observations	752391	8284	59146
Number of reflections	57469	807	4402
<I/σ(I)>	19.8	64	1.8
Completeness [%]	100.0	99.3	100
Redundancy	13.1	10.3	13.4
CC(1/2)	0.999	1.000	0.579

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M NaI, 1.6 M ammonium sulfate, 50 mM Tris 8.5