6B8J

Co-structure of human glycogen synthase kinase beta with a selective (5-imidazol-2-yl-4-phenylpyrimidin-2-yl)[2-(2-pyridylamino)ethyl]amine inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-01-15
Detector	RIGAKU RAXIS II
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	56.747, 65.070, 57.336
Unit cell angles	90.00, 100.53, 90.00

Refinement procedure

Resolution	25.000 - 2.595
R-factor	0.2155
Rwork	0.212
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.595
Number of reflections	12763
<I/σ(I)>	12.4
Completeness [%]	99.7
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	7-12% (w:v) PEG 6000 and 5-8% MPD (v:v)