6B7H
Structure of mGluR3 with an agonist
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-12 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 0.97973 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.081, 80.081, 161.887 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.280 - 2.820 |
| R-factor | 0.1852 |
| Rwork | 0.184 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | SCALA |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.280 | |
| High resolution limit [Å] | 2.820 | 2.820 |
| Number of reflections | 15043 | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.0 | |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 100 mM Bis-Tris, pH 5.5, 17% PEG10000, 100 mM ammonium acetate |
