6B6F
Beta-Lactamase, mixed with Ceftriaxone, needles crystal form, 2sec
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 300 |
Detector technology | PIXEL |
Collection date | 2016-09-10 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 1.3 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.730, 41.690, 69.530 |
Unit cell angles | 90.00, 104.68, 90.00 |
Refinement procedure
Resolution | 19.854 - 2.050 |
R-factor | 0.2023 |
Rwork | 0.200 |
R-free | 0.25030 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.573 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.854 |
High resolution limit [Å] | 2.050 |
Number of reflections | 13311 |
<I/σ(I)> | 5.1 |
Completeness [%] | 100.0 |
Redundancy | 238 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | free interface diffustion | 293 | 0.1 M sodium acetate pH 5.0, 45% PEG 1000 |