6B65

IMPase (AF2372) R92Q/K164E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-08-31
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	90.315, 90.315, 101.412
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.480 - 2.702
R-factor	0.2006
Rwork	0.198
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lvb
RMSD bond length	0.018
RMSD bond angle	1.236
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.480
High resolution limit [Å]	2.700
Number of reflections	24448
<I/σ(I)>	2.776
Completeness [%]	96.5
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100 mM myo inositol 14% PEG 3350 200 mM potassium sulfate 100 mM magnesium chloride 100 mM Hepes 7.0