6B5T
Structure of PfCSP peptide 29 with human antibody CIS42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.580, 70.664, 163.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.055 - 2.222 |
R-factor | 0.1845 |
Rwork | 0.183 |
R-free | 0.20910 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.799 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.055 |
High resolution limit [Å] | 2.220 |
Number of reflections | 24551 |
<I/σ(I)> | 31.1 |
Completeness [%] | 99.6 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG3350, 9% Isopropanol, 0.12M Ammonium Citrate pH 8.5 |