6B5Q

DCN1 bound to 38

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9787
Spacegroup name	C 2 2 21
Unit cell lengths	88.087, 90.841, 105.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.440 - 2.160
R-factor	0.197
Rwork	0.195
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.990
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.200
High resolution limit [Å]	2.160	5.860	2.160
Rmerge	0.048	0.028	0.226
Rmeas	0.052	0.031	0.244
Rpim	0.019	0.012	0.090
Total number of observations	168961
Number of reflections	22799	1197	1113
<I/σ(I)>	15.5
Completeness [%]	99.8	96.5	100
Redundancy	7.4	6.6	7.3
CC(1/2)		0.999	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	37 % PEG 300, 0.1 M phosphate-citrate pH 4.2