6B5L
Structure of PfCSP peptide 20 with human protective antibody CIS43
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.942, 61.668, 75.371 |
Unit cell angles | 90.00, 106.04, 90.00 |
Refinement procedure
Resolution | 45.140 - 2.400 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.826 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.142 |
High resolution limit [Å] | 2.400 |
Number of reflections | 15363 |
<I/σ(I)> | 6.1 |
Completeness [%] | 93.3 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Ammonium Sulfate, 0.1M Sodium 659 Acetate Trihydrate pH 4.6, 30% PEG MME 2000 |