6B58
FrdA-SdhE assembly intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.640, 63.330, 175.580 |
| Unit cell angles | 90.00, 96.83, 90.00 |
Refinement procedure
| Resolution | 37.153 - 2.611 |
| R-factor | 0.1946 |
| Rwork | 0.191 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kf6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.068 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 41620 |
| <I/σ(I)> | 11.9 |
| Completeness [%] | 96.8 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 90 mM Bis-Tris pH 5.5, 100 mM NH4CH3COO, 20% PEG 10,000 and 50mM NaMalonate |
