6B4E

Crystal structure of Saccharomyces cerevisiae Gle1 CTD-Nup42 GBM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	67.470, 67.470, 361.651
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.299 - 1.750
R-factor	0.1855
Rwork	0.185
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rrn
RMSD bond length	0.007
RMSD bond angle	1.111
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2006: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	1.810
High resolution limit [Å]	1.750	1.750
Rpim	0.025	0.398
Number of reflections	85335	7866
<I/σ(I)>	16.4	1.3
Completeness [%]	99.3	93.5
Redundancy	21.8	10.5
CC(1/2)	0.999	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M HEPES pH 8.2, 11 % (w/v/) PEG 3350, 0.2 M L-Proline