6B2N
Crystal structure of TEM-1 beta-lactamase mutant M182N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-04-03 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.910, 49.660, 122.160 |
Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
Resolution | 19.767 - 2.000 |
R-factor | 0.2347 |
Rwork | 0.231 |
R-free | 0.28280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jwp |
RMSD bond length | 0.003 |
RMSD bond angle | 0.582 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 66091 | |
<I/σ(I)> | 8.95 | |
Completeness [%] | 98.6 | |
Redundancy | 3.52 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000 |