6B27
Crystal structure of human STAC2 Tandem SH3 Domains (296-411) in complex with a CaV1.1 II-III loop peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.920, 114.650, 144.678 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 1.730 |
R-factor | 0.1804 |
Rwork | 0.179 |
R-free | 0.21630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.935 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 1.780 |
High resolution limit [Å] | 1.730 | 3.960 | 1.730 |
Rmerge | 0.058 | 0.023 | 0.493 |
Rmeas | 0.068 | 0.027 | 0.577 |
Rpim | 0.035 | 0.014 | 0.294 |
Total number of observations | 301764 | ||
Number of reflections | 83341 | 7304 | 6866 |
<I/σ(I)> | 7.8 | ||
Completeness [%] | 99.1 | 98.5 | 99.6 |
Redundancy | 3.6 | 3.5 | 3.6 |
CC(1/2) | 0.998 | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 277.15 | 2.24M ammonium sulfate, 0.1M sodium acetate |