6B1N
Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | .977 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.388, 77.598, 75.523 |
Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
Resolution | 37.048 - 1.800 |
R-factor | 0.1854 |
Rwork | 0.184 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6b1i |
RMSD bond length | 0.012 |
RMSD bond angle | 1.193 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.300 |
High resolution limit [Å] | 1.700 |
Number of reflections | 87978 |
<I/σ(I)> | 7.4 |
Completeness [%] | 96.3 |
Redundancy | 4.3 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | NaCl, Tris, PEG 3350, MgCl2, ADP |