6B0W
Crystal structure of the anti-circumsporozoite protein 1710 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-16 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.063, 66.724, 124.027 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.424 - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gsd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.787 |
| Data reduction software | XDS |
| Data scaling software | XPREP |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.424 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.044 | 0.289 |
| Number of reflections | 40776 | 5691 |
| <I/σ(I)> | 13.2 | 1.9 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 17 % (w/v) PEG 400, 15 % (v/v) glycerol, 8.5 % (v/v) isopropanol and 85 mM HEPES pH 7.5 |
