6B0R
Zinc finger Domain of WT1(-KTS form) with M342R Mutation and 14+1mer Oligonucleotide with 3' Triplet TGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-02 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.940, 35.113, 87.148 |
| Unit cell angles | 90.00, 110.03, 90.00 |
Refinement procedure
| Resolution | 31.321 - 1.818 |
| R-factor | 0.2092 |
| Rwork | 0.208 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.463 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.350 | 1.920 |
| High resolution limit [Å] | 1.818 | 1.850 |
| Rmerge | 0.121 | 0.959 |
| Rpim | 0.030 | 0.301 |
| Number of reflections | 39284 | 3843 |
| <I/σ(I)> | 21.3 | 3 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 17.4 | 9.4 |
| CC(1/2) | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 30% PEG 2000 MME o.1M KCN |
