6B0Q
Zinc finger Domain of WT1(-KTS form) with 13+1mer Oligonucleotide with 3' Triplet TGT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-18 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 161.664, 161.664, 42.172 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.830 - 2.794 |
| R-factor | 0.2262 |
| Rwork | 0.225 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.479 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.830 | 2.790 |
| High resolution limit [Å] | 2.790 | 2.680 |
| Rmerge | 0.114 | 0.822 |
| Rpim | 0.051 | 0.394 |
| Number of reflections | 30219 | 1516 |
| <I/σ(I)> | 13.5 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.9 | 5.4 |
| CC(1/2) | 0.686 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 25% PEG 3350 0.2M Lithium Sulfate 0.1M BIS-TRIS |
