6B0H
Crystal structure of Pfs25 in complex with the transmission blocking antibody 1262
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-14 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.110, 119.010, 165.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.262 - 2.700 |
R-factor | 0.1889 |
Rwork | 0.186 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z27 1262 Fab |
RMSD bond length | 0.004 |
RMSD bond angle | 0.649 |
Data reduction software | XDS |
Data scaling software | XPREP |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.262 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rpim | 0.089 | 0.434 |
Number of reflections | 36020 | 3525 |
<I/σ(I)> | 9.7 | 2.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M magnesium acetate and 20% (w/v) PEG 3350 |