6B0A
Crystal structure of Pfs25 in complex with the transmission blocking antibody 1269
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.100, 39.503, 101.845 |
| Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
| Resolution | 39.073 - 2.500 |
| R-factor | 0.2189 |
| Rwork | 0.217 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z27 1269 Fab |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.467 |
| Data reduction software | XDS |
| Data scaling software | XPREP |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.030 | 0.294 |
| Number of reflections | 23042 | 2539 |
| <I/σ(I)> | 17.4 | 2.1 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M di-ammonium hydrogen citrate, 20 % (w/v) PEG 3350 |
