6B08
Crystal structure of Pfs25 in complex with the transmission blocking antibody 1276
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.351, 70.264, 83.742 |
| Unit cell angles | 90.00, 90.45, 90.00 |
Refinement procedure
| Resolution | 38.748 - 2.200 |
| R-factor | 0.1927 |
| Rwork | 0.191 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1276 Fab 1z27 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.689 |
| Data reduction software | XDS |
| Data scaling software | XPREP |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rpim | 0.033 | 0.300 |
| Number of reflections | 40434 | 5096 |
| <I/σ(I)> | 16.3 | 2 |
| Completeness [%] | 98.0 | 99.4 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M di-ammonium hydrogen phosphate, 18 % (w/v) PEG 3350 |
